Line-Gene 9600 Plus is the newest product of Bioer's Real-Time PCR detection system family. Based on Line-Gene 9600’s large sample capacity, wide temperature range and multi-channels, Line-Gene 9600 Plus provides brand-new & humanized running and operation interface for better operating experience. Line-Gene 9600 Plus adopts Ferrotec specially-made Peltier, advanced fiber optic technology, new global wide-range power supply, the unique patented block dissipation technology and bottom detection mode, which provides better heating/cooling rate, temperature accuracy, uniformity and stability. Line-Gene 9600 Plus has up to 96 sample capacity and fast speed of dual-color scanning, and the design of 5 channel detection also covers the whole fluorescence detection wavelength. Newly-designed automatic hot-lid without manual operation ensures constant pressure for various tube heights. This auto hot-lid mode could be easily integrated into various automatic systems. Its Windows tablet computer and new touch screen brings excellent operating experience. Newly-improved Line-Gene 9600 Plus Real-Time PCR detection system can satisfy various clients' requirement either for research or clinical use.

Line-Gene 9600 Plus is the newest product of Bioer's Real-Time PCR detection system family. Based on Line-Gene 9600’s large sample capacity, wide temperature range and multi-channels, Line-Gene 9600 Plus provides brand-new & humanized running and operation interface for better operating experience. Line-Gene 9600 Plus adopts Ferrotec specially-made Peltier, advanced fiber optic technology, new global wide-range power supply, the unique patented block dissipation technology and bottom detection mode, which provides better heating/cooling rate, temperature accuracy, uniformity and stability. Line-Gene 9600 Plus has up to 96 sample capacity and fast speed of dual-color scanning, and the design of 4 channel detection also covers the whole fluorescence detection wavelength. Newly-designed automatic hot-lid without manual operation ensures constant pressure for various tube heights. This auto hot-lid mode could be easily integrated into various automatic systems. Its Windows tablet computer and new touch screen brings excellent operating experience. Newly-improved Line-Gene 9600 Plus Real-Time PCR detection system can satisfy various clients' requirement either for research or clinical use.

Bioprep-24 is your solution for sample preparation and cell lysis. The reliable, fast and high-throughput homogenizer to extract DNA, RNA or proteins from aniaml, human and plant tissues, micro-organisms, spores, bones, etc. Grind and homogenize a large range of biological samples, compatible with any analysis DNA-RNA Electrophoresis, Liquid Chromatography, Western Blot etc.Bioprep-24 is your solution for sample preparation and cell lysis. The reliable, fast and high-throughput homogenizer to extract DNA, RNA or proteins from aniaml, human and plant tissues, micro-organisms, spores, bones, etc. Grind and homogenize a large range of biological samples, compatible with any analysis DNA-RNA Electrophoresis, Liquid Chromatography, Western Blot etc.Bioprep-24 is your solution for sample preparation and cell lysis. The reliable, fast and high-throughput homogenizer to extract DNA, RNA or proteins from aniaml, human and plant tissues, micro-organisms, spores, bones, etc. Grind and homogenize a large range of biological samples, compatible with any analysis DNA-RNA Electrophoresis, Liquid Chromatography, Western Blot etc.Bioprep-24 is your solution for sample preparation and cell lysis. The reliable, fast and high-throughput homogenizer to extract DNA, RNA or proteins from aniaml, human and plant tissues, micro-organisms, spores, bones, etc. Grind and homogenize a large range of biological samples, compatible with any analysis DNA-RNA Electrophoresis, Liquid Chromatography, Western Blot etc.

Shaking Water Baths Shaking Water Baths (also known as Reciprocating Water Baths), are designed to handle a wide variety of applications. These baths can be used effectively in molecular biology protocols (such as hybridization), bacterial culturing, as well as in solubility and metabolism studies. The SHEL LAB Models SWBR17 and SWBR27 Reciprocating Water Baths are designed to deliver precise temperature control and a smooth reciprocal shaking motion. The oscillator control is independent, allowing the baths to also be used for regular constant temperature water bath applications such as thawing, warming reagents, or general incubation. Both the SWBR17, and SWBR27 Shaking Water Baths offer an adjustable stroke length ( 0.5″, 1″ or 1.5″), giving the user the ability to affect the degree of agitation. With a RPM range of 20-200, these versatile reciprocating water baths are sure to meet many application needs. Shaking Water Bath Applications Hybridization Applications, Cell Cultures, Cell Aeration, Increasing Solubility Rates, Molecular Biology Assays, Bacterial Cultures.

Fast, effective and reproducible homogenization of a wide range of samples, including tissues and cells hard to lyse. They also own extraordinary power for the processing of even impact resistant samples such as bones or cartiage. The Bioprep-6 are ideal solutions for releasing DNA, RNA, Proteins, Enzymes, etc. from very tough samples while still retaining molecular integrity  

INCUBATORS, NATURAL-FLOW .Variable Application: Microorganism Culture, Animal & Plant Cell .Culture, Constant Temperature, Germination, and so on .Optimized Air Circulation by Natural Flow Preventing Cross .Contamination of Samples .Locking Mode Supported for Experimental Safety (Input to Jog-.Shuttle can be Disabled) .RS232C Interface for Monitoring and Controlling with PC .PL (Product Liability) Insurance .UL/CUL Certified & CE Certified

Maintenance free brushless DC motor, Max. speed 1100rpm, Max. temperature 120C, Stainless steel work plate, cover with silicone cushion, provides the execellent performance of heating uniformity and skid resistance.

High quality motor providing excellent precision and accuracy, Electronic control decreases the repetitive strain injury, the remove control panel makes the operating process without the manual disturbance, Build-in magnetic stirrer support.

LCD Display for settings and monitoring of speed and temperature, Bult-in PID controller ensures the safe heating of the medium with overheating protection, glass ceramic work plate provides excellent chemical resistant performance and most eifficient heat transfer, Outstanding brushless DC motor performance enables more stirring power, two rotating knobs enable the easy ane direct chage of speed and temperature

  Three key factors-ergonomics, user-friendliness and reliability – form the cornerstone of our product development. DLAB dPette Electronic Pipette is an excellent example of combining all of these aspects in one product, a valued instrument for professionals who strived for high quality results.

  Three key factors-ergonomics, user-friendliness and reliability – form the cornerstone of our product development. DLAB dPette Electronic Pipette is an excellent example of combining all of these aspects in one product, a valued instrument for professionals who strived for high quality results.

      Three key factors-ergonomics, user-friendliness and reliability –form the cornerstone of our product development.DLAB dPette Electronic Pipette is an excellent example ofcombining all of these aspects in one product, a valued instrument for professionals who strived for high quality results.

ชุดสกัดสารพันธุกรรม RNA จากเซลล์และเนื้อเยื่อสัตว์ สกัดได้ 50 ตัวอย่าง เป็นชุดสกัดแบบ Spin Column

ชุดสกัดสารพันธุกรรม RNA จาก cultured cells, animal tissues และ plant tissues สกัดได้ 200 ตัวอย่าง เป็นชุดสกัดแบบ Spin Column

Electronic pipette dPette Three key factors-ergonomics, user-friendliness and reliability – form the cornerstone of our product development. DLAB dPette Electronic Pipette is an excellent example of combining all of these aspects in one product, a valued instrument for professionals who strived for high quality results. • Comprehensive range of pipetting modes for speed of use • Three iPette Electronic Pipette working modes, Pipette , Stepper Pipette,Dispenser Pipette • Enhanced motor concept with built-in error control providing outstanding accuracy and reliability • Li-ion battery enabling longer operation time on each charge • 3 speeds for aspiration and dispensing • Easy charging-stand charging or direct charging • 121℃ Autoclavable low-half part  

Electronic pipette dPette Three key factors-ergonomics, user-friendliness and reliability – form the cornerstone of our product development. DLAB dPette Electronic Pipette is an excellent example of combining all of these aspects in one product, a valued instrument for professionals who strived for high quality results. • Comprehensive range of pipetting modes for speed of use • Three iPette Electronic Pipette working modes, Pipette , Stepper Pipette,Dispenser Pipette • Enhanced motor concept with built-in error control providing outstanding accuracy and reliability • Li-ion battery enabling longer operation time on each charge • 3 speeds for aspiration and dispensing • Easy charging-stand charging or direct charging • 121℃ Autoclavable low-half part

Electronic pipette dPette Three key factors-ergonomics, user-friendliness and reliability – form the cornerstone of our product development. DLAB dPette Electronic Pipette is an excellent example of combining all of these aspects in one product, a valued instrument for professionals who strived for high quality results. • Comprehensive range of pipetting modes for speed of use • Three iPette Electronic Pipette working modes, Pipette , Stepper Pipette,Dispenser Pipette • Enhanced motor concept with built-in error control providing outstanding accuracy and reliability • Li-ion battery enabling longer operation time on each charge • 3 speeds for aspiration and dispensing • Easy charging-stand charging or direct charging • 121℃ Autoclavable low-half part

Electronic pipette dPette Three key factors-ergonomics, user-friendliness and reliability – form the cornerstone of our product development. DLAB dPette Electronic Pipette is an excellent example of combining all of these aspects in one product, a valued instrument for professionals who strived for high quality results. • Comprehensive range of pipetting modes for speed of use • Three iPette Electronic Pipette working modes, Pipette , Stepper Pipette,Dispenser Pipette • Enhanced motor concept with built-in error control providing outstanding accuracy and reliability • Li-ion battery enabling longer operation time on each charge • 3 speeds for aspiration and dispensing • Easy charging-stand charging or direct charging • 121℃ Autoclavable low-half part

ชุดสกัดสารพันธุกรรม DNA จากตัวอย่างเลือด , เซลล์ , เนื้อเยื่อ , แบคทีเรีย สกัดได้ 50 ตัวอย่าง เป็นชุดสกัดแบบ Spin Column

ROCKING MIXER  It's a machine who used for rocking, structure, the range of oscillation is small, the frequency is adjustable, it's used for running gel separate spectral band, fixing, rocking when dyeing, crock for Coomassie blue, fixing, dyeing, developing when dyeing of silver nitrate. It's used for curing after  X-ray negative film, cell culture and blood mixer.

Spin Columns DNA Extraction Kits.

• Fully autoclavable Ergonomic design provides excellent operating  experience  Easy-to-read  volume display.The pipettes cover the volume range from 0.1μL to 5mL Easy calibration and maintenance  Manufactured from innovative materials  Each MicroPette Plus supplied with anindividual calibration certificate according to ISO8655

 Nano-300  is an advanced model of Micro UV VIS Spectrophotometer based on Nano-100, with full range of wavelength (200-800nm). It is with an added new function of bacterium cell concentration measurement (OD600) in a cuvette. The Nano-300 only requires 0.5-2ul sample to measure nucleic acids, protein as quickly like Nano-100 do. Nano-300 come with a 7 inch touch screen and integranted Android operating system, with no computer required. It is an ideal equipment for a biology laboratory to make life science research more efficient.

CO2 Incubators The SHEL LAB CO2 Incubator family includes the following design features and the longest warranty period on the market – a five year limited warranty on most models: Infrared (IR) CO2 Sensor is extremely quick to recover CO2 levels after door openings and ensures superior pH stability Unique air jacket design provides excellent temperature uniformity of +/-0.25°C at 37°C Humidity levels, maintained through evaporation, of up to 95% Data logging capabilties

Shaking Water Baths Shaking Water Baths (also known as Reciprocating Water Baths), are designed to handle a wide variety of applications. These baths can be used effectively in molecular biology protocols (such as hybridization), bacterial culturing, as well as in solubility and metabolism studies. The SHEL LAB Models SWBR17 and SWBR27 Reciprocating Water Baths are designed to deliver precise temperature control and a smooth reciprocal shaking motion. The oscillator control is independent, allowing the baths to also be used for regular constant temperature water bath applications such as thawing, warming reagents, or general incubation. Both the SWBR17, and SWBR27 Shaking Water Baths offer an adjustable stroke length ( 0.5″, 1″ or 1.5″), giving the user the ability to affect the degree of agitation. With a RPM range of 20-200, these versatile reciprocating water baths are sure to meet many application needs. Shaking Water Bath Applications Hybridization Applications, Cell Cultures, Cell Aeration, Increasing Solubility Rates, Molecular Biology Assays, Bacterial Cultures.

Reliable heating/cooling elements and innovative temperature control, High performance DSP and temperature control, Excellent temperature uniformity, high precise temperature control, Fast heating and cooling rate, 7 inch Large color touch panel screen for easy programming, large user program storage.

Gene Touch GeneTouch is the new PCR multifunctional instrument where the company meticulously. GeneTouch with its excellent and powerful function to ensure the reliable results and stable. The new password protection function and the memory function to meet customer requirements for data security.

Digital display for both rotation speed and heating temperature allows for optimal controlling of all distillation processes, Motor lift automatic release the evaporating flask to a safety position in case of power failure, Wide temperature range of the 5ml heating bath(Room temperature 180C) Overheating protection temperature at 220C, Speed range from 20 to 280 rpm,. and inteval operation in clockwise and anticlockwise directions for drying process, Patented condenser with excellent cooling effect, ejection mechanism ensures easy removal of evaporating flask, patented double spring sealing ring which made of PTFE provides an excellent sealing performance, Remote function provides PC control and data transmission.

Reliable heating/cooling elements and innovative temperature control, High performance DSP and temperature control, Excellent temperature uniformity, high precise temperature control, Fast heating and cooling rate, 7 inch Large color touch panel screen for easy programming, large user program storage.

         The spectrophotometer is an indispensable instrument routinely used in colleges, universities and research institutes for quantitative analysis in fields including biotechnology and quality control of new material developed. V-Series provides high resolution, high performance and excellent stability.

Maintenance-free brushless DC motor, speed range of 0-1100rpm, Stainless steel work plate cover with silicone cushion, provide excellent performance of skid resistance.

 SAFEVAC : Vacuum Aspiration System    SAFEVAC Vacuum Aspiration System is idealfor aspiration of liquid and their subsequent disposal, and widely used in the removal of media from cell cultures, draw the supernatants after centrifugation or the wash solutions from immune-assay well-plates, etc, providing the safety first for the laboratory.

-External material : excellent cool-rolled steel, coating static. -Stainless steel inner chamber, round angle structure, adjustable shelves. -Double-layer glass observation window, adjustable airtight buckle lock. -Over temperature protection.

-External material : excellent cool-rolled steel, coating static. -Stainless steel inner chamber, round angle structure, adjustable shelves. -Double-layer glass observation window, adjustable airtight buckle lock. -Over temperature protection.

Capable of holding single or double microplates, the compact shape of MX-M Microplate mixer takes less bench space and makes storage easier, It is widely used in life science including microbiology, cell and molecular biology, immunology and biotechnology, A wide range of speed from 0 to 1500rpm, Used for various mixing applications with optional adapters, Maintenance-free brushless DC motor.

Lead Fluid silicone tubing adopts imported silicone rubber material and platinum-cured process. The tubing has characteristics such as non-toxic, biological inert and UV resistance, ozone resistance, heat resistance, high transparency, strong resilience, resistance to compression, etc. It is the optimal material of tubing and can be widely used in scientific research and production areas such as GMP pharmacy, biotechnology, molecular biology, fine chemistry, environmental protection and food processing. Excellent durability and aging resistance; outstanding physical and mechanical properties; weak performance in acid and solvent resistance. High anti-break intensity; good transparency; excellent physical and biologic inertia. Water pressure resistance：4-8bar

Lead Fluid silicone tubing adopts imported silicone rubber material and platinum-cured process. The tubing has characteristics such as non-toxic, biological inert and UV resistance, ozone resistance, heat resistance, high transparency, strong resilience, resistance to compression, etc. It is the optimal material of tubing and can be widely used in scientific research and production areas such as GMP pharmacy, biotechnology, molecular biology, fine chemistry, environmental protection and food processing. Excellent durability and aging resistance; outstanding physical and mechanical properties; weak performance in acid and solvent resistance. High anti-break intensity; good transparency; excellent physical and biologic inertia. Water pressure resistance：4-8bar

            Excellent chemical resistance, components are made of (PTFE, FEP, BSG, PP), Fully autoclavable at 121C, Four ranges of bottle-top dispenser covering a volume range from 0.5ml to 50ml, Easy for cleaning and maintenance, the Optional flexible discharge tube with safety handle permits fast and precise dispensing, Dispensemate is supplied with S40, GL32, GL38, GL25,GL28 sized adapters sized adaptors.

Cell Culture Plate (จานเพาะเชื้อ - จานเลี้ยงเชื้อ - เพลทเลี้ยงเชื้อ) 100pcs/box, individual 96 well, Flat bottom TC-treated surfaces Sterile  

Cell Culture Plate (จานเพาะเชื้อ - จานเลี้ยงเชื้อ - เพลทเลี้ยงเชื้อ) 100pcs/box, individual 6 well, Flat bottom TC-treated surfaces Sterile

Cell Culture Plate (จานเพาะเชื้อ - จานเลี้ยงเชื้อ - เพลทเลี้ยงเชื้อ) 100pcs/box, individual 12 well, Flat bottom TC-treated surfaces Sterile

Cell Culture Plate (จานเพาะเชื้อ - จานเลี้ยงเชื้อ - เพลทเลี้ยงเชื้อ) 100pcs/box, individual 24 well, Flat bottom TC-treated surfaces Sterile

Cell Culture Plate (จานเพาะเชื้อ - จานเลี้ยงเชื้อ - เพลทเลี้ยงเชื้อ) 100pcs/box, individual 48 well, Flat bottom TC-treated surfaces Sterile

Cell Culture Plate (จานเพาะเชื้อ - จานเลี้ยงเชื้อ - เพลทเลี้ยงเชื้อ) 100pcs/box, individual 96 well, U bottom TC-treated surfaces Sterile

Lead Fluid silicone tubing adopts imported silicone rubber material and platinum-cured process. The tubing has characteristics such as non-toxic, biological inert and UV resistance, ozone resistance, heat resistance, high transparency, strong resilience, resistance to compression, etc. It is the optimal material of tubing and can be widely used in scientific research and production areas such as GMP pharmacy, biotechnology, molecular biology, fine chemistry, environmental protection and food processing. Excellent durability and aging resistance; outstanding physical and mechanical properties; weak performance in acid and solvent resistance. High anti-break intensity; good transparency; excellent physical and biologic inertia. Water pressure resistance：4-8bar

Maintenance fee brushless DC motor, rare earth magnets give powerful stirring, Stepless speed regulation 300-2000rpm, Ultra silence and vibration-fee design, PET top provides excellent chemical resistance, Shaft can be fitted with a PH sensor or sensors for other purpose.

Lead Fluid silicone tubing adopts imported silicone rubber material and platinum-cured process. The tubing has characteristics such as non-toxic, biological inert and UV resistance, ozone resistance, heat resistance, high transparency, strong resilience, resistance to compression, etc. It is the optimal material of tubing and can be widely used in scientific research and production areas such as GMP pharmacy, biotechnology, molecular biology, fine chemistry, environmental protection and food processing. Excellent durability and aging resistance; outstanding physical and mechanical properties; weak performance in acid and solvent resistance. High anti-break intensity; good transparency; excellent physical and biologic inertia. Water pressure resistance：4-8bar

Lead Fluid silicone tubing adopts imported silicone rubber material and platinum-cured process. The tubing has characteristics such as non-toxic, biological inert and UV resistance, ozone resistance, heat resistance, high transparency, strong resilience, resistance to compression, etc. It is the optimal material of tubing and can be widely used in scientific research and production areas such as GMP pharmacy, biotechnology, molecular biology, fine chemistry, environmental protection and food processing. Excellent durability and aging resistance; outstanding physical and mechanical properties; weak performance in acid and solvent resistance. High anti-break intensity; good transparency; excellent physical and biologic inertia. Water pressure resistance：4-8bar

Bacteria culture dish, จานเพาะเชื้อ, จานเลี้ยงเชื้อ 90*15mm Sterile 500pc/case

Red Blood Cell Lysis Buffer was developed to lyse red blood cells of human whole blood. The buffer provides a very simple, fast economic way to isolate white blood cells from whole blood without destroying the white blood cells. In general, with the buffer, you can get 80% or more white blood cells from the sample.

Material: LDPE Ideal for use in blood banking, urinalysis, hematology and microbiology Eliminates the hazard of damage and exposure to infectious materials Low affinity surface reduces the loss of cells and valuable proteins

Material: LDPE Ideal for use in blood banking, urinalysis, hematology and microbiology Eliminates the hazard of damage and exposure to infectious materials Low affinity surface reduces the loss of cells and valuable proteins

Material: LDPE Ideal for use in blood banking, urinalysis, hematology and microbiology Eliminates the hazard of damage and exposure to infectious materials Low affinity surface reduces the loss of cells and valuable proteins

Material : PMP Jar/ PP closure - Autoclavable Offers clarity and good chemical resistance  Excellent for use in museum, formaldehyde loss is only 2% per year

Beaker Griffin Low - Form Made from PMP Easy to read silk screened graduations Double graduated metric scale Offers excellent chemical resistance Unsuitable for use on ahot plate  Stackable Autoclavable Higher transparency, chemical resistance and heat tolerant than PP

Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is ideal for isolating PCR and RT-PCR templates. The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 μg/mL for protein removal and enzyme inactivation and up to 2 mg/mL for tissue treatment. Proteinase K products are free of detectable DNase and RNase.

TRIsure provides a simple, efficient method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials. By combining thiocyanate compounds with phenol, TRIsure facilitates disruption of cells during homogenization and effectively inhibits DNase and RNase activity. After homogenizing the sample with TRIsure, chloroform is added and the homogenate is allowed to separate into three phases: a clear aqueous phase (upper) containing the RNA, a green colored organic phase (lower) and an interphase, containing the DNA and proteins. The RNA is extracted from the aqueous phase by isopropyl alcohol precipitation, DNA is precipitated from the organic layer with ethanol and the proteins are precipitated from the phenol-ethanol supernatant by isopropyl alcohol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for use in downstream applications. TRIsure allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population. The simplicity of the TRIsure method allows simultaneous processing of a large number of samples and can be used in a wide variety of downstream applications including expression profiling, epigenetics, genotyping, transgenic analysis and cell line characterization.

Product Highlights Excellent clarity - enhances visualization, even at high concentrations Strong gels at low concentration - better handling, less breakage DNase/RNase-free - for all nucleic acid separation Product Description Extremely pure, high molecular biology grade Agarose from Bioline has no detectable DNase or RNase activity and forms strong gels with low backgrounds. Due to its low EEO, DNA will have a high electrophoretic mobility.

Product Highlights Induces E.coli lac operon activity - suitable for cloning and protein expression procedures Dioxane free - does not disrupt normal cell function >99.6% purity - confirmed by HPLC Convenient - available as powder and stabilized stock solution Product Description Isopropyl-ß-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme ß--Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG. IPTG is part of the Bioline Essentials range of products, which includes agaroses, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.

The ISOLATE II Plant DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of plant materials, without the need for hazardous reagents such as phenol. By combining the stringency of CTAB lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plant DNA Kit provides a fast method for the purification of high-quality genomic DNA from most plant cells, particularly those rich in polysaccharides, including leaves, bark, roots and fruits as well as dung, animal-fecal, soil and compost samples. Some plant tissues do not lyse well in CTAB and so an SDS-based lysis buffer is also provided as an alternative. Residual amounts of RNA remaining can be removed using an RNase treatment during lysis with RNase A that is supplied with the kit.  The ISOLATE II Plant DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR with any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase                                     "Isolating DNA from soil samples is a big challenge because samples are full of inhibitors like polymers or low-molecular substances, which inhibit downstream applications. Therefore, it is difficult to find a kit which can deliver stable DNA. The ISOLATE II Plant DNA Kit is able to handle these kinds of samples and worked well for soil samples." Katharina Schulte, Technical College Zweibruecken, Germany

The ISOLATE II RNA Mini Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol. By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Mini Kit provides a fast method for the purification of high-quality total RNA from animal and plant cells and tissues as well as cultured cells, bacterial cells, yeast, biological fluids and cell-free samples. Biological samples which are sometimes difficult to process i.e. mouse tissue (liver, brain), various tumor cell lines, Streptococcus and Actinobacillus pleuropneumoniae, will yield high-quality RNA with the ISOLATE II RNA Mini Kit. The online product manual has protocols for purifying total RNA from cultured cells, tissues, yeast, bacteria, biological liquids, paraffin embedded tissue and RNAlater® treated samples. There is also a protocol for a convenient on-column DNase treatment, using RNase-free DNase I that is supplied with the kit, for applications that are sensitive to very small amounts of DNA. The ISOLATE II RNA Mini Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Mini Kit can be used to purify samples for PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.

The ISOLATE II RNA Plant Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of plant materials, including leaves, bark, roots and fruits, without the need for hazardous reagents such as phenol. By combining the stringency of guanidinium thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Plant Kit provides a fast method for the purification of high-quality total RNA from most plant cells, including plant tissues, leaves, bark, roots and fruits. Some plant tissues such as maize endosperm and the mycelia of filamentous fungi do not lyse well in guanidinium thiocyanate and can solidify, resulting in poor yields and so guanidinium hydrochloride lysis buffer is also provided as an alternative. Residual amounts of contaminating DNA are selectively removed using a column, however for ultrasensitive applications, remaining DNA can be removed using RNase-free DNase I that is supplied with the kit.  The ISOLATE II RNA Plant Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Plant Kit can be used to purify samples prior to RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.

The ISOLATE II miRNA Kit provides a simple, efficient column-based method for the isolation of miRNA and total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol, which has been reported to introduce significant bias in recovery of selected small RNAs. By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of two silica-membrane purification columns, the ISOLATE II miRNA Kit provides a fast method for the purification of high-quality total RNA (on the first column) and miRNA (on the second column) from cultured animal cells, small tissue samples, bacterial cells, plants and blood. For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment with RNase-free DNase I that is supplied with the kit. The ISOLATE II miRNA Kit has been designed to deliver optimal performance with the EPIK Panel and Select Assays in miRNA expression profiling and quantification using qPCR, microarrays, small RNA library construction for small RNA-Seq, as well as single cell assays.

The ISOLATE II RNA/DNA/Protein Kit provides a simple, efficient column-based method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials, without the need for hazardous reagents such as phenol. By combining the stringency of guanidine-thiocyanate lysis with the speed and ease-of-use of three silica-membrane purification columns, the ISOLATE II RNA/DNA/Protein Kit provides a fast method for the purification of high-quality total RNA, genomic DNA and proteins from a single sample of cultured cells, mammalian tissue, biofluids (including saliva, urine, semen and blood), bacteria, yeast, fungi or plants. For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment with RNase-free DNase I that is supplied with the kit.  The ISOLATE II RNA/DNA/Protein Kit allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population. The purified nucleic acids and proteins are of the highest purity and integrity and the ISOLATE II RNA/DNA/Protein Kit has been designed to deliver optimal performance in a wide variety of downstream applications including miRNA profiling using the EPIK Panel and Select Assays, novel biomarker discovery, siRNA gene silencing studies, gene expression profiling, as well as epigenetics, genotyping, transgenic analysis and cell line characterization.

MyTaq™ Red Mix is recommended for all standard PCR applications. MyTaq Red Mix is comprised of MyTaq DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq means it gives excellent results under fast PCR conditions. The product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up, for improved speed, throughput and assay reproducibility. MyTaq Red Mix contains a red dye that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, the MyTaq red reaction buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

MyTaq HS Red Mix is recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. MyTaq HS Red Mix is comprised of MyTaq HS DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq HS has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors.  Furthermore, the highly efficient nature of MyTaq HS means it gives excellent results under fast PCR conditions. MyTaq HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start. The product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up, for improved speed, throughput and assay reproducibility. MyTaq Red Mix contains a red dye that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, MyTaq Red contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

The SensiFAST Probe No-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. SensiFAST Probe One-Step has been optimized to deliver excellent results in both singleplex and multiplex assays. An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation. The SensiFAST™ Probe No-ROX One-Step Kit has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX. SensiFAST Probe One-Step has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes.                                                     "I compared the SensiFAST Probe One-Step Kit with another very popular product. SensiFAST Probe One-Step gave improved sensitivity, which was reflected in lower Ct values. Also, this product is a very good price. Value for money!!" Peony Fung, Cerberus Sciences, Thebarton, Australia

The SensiFAST Probe Lo-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. SensiFAST Probe One-Step has been optimized to deliver excellent results in both singleplex and multiplex assays. An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation. The SensiFAST™ Probe Lo-ROX One-Step Kit has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX. SensiFAST Probe One-Step has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes. "I compared the SensiFAST Probe One-Step Kit with another very popular product. SensiFAST Probe One-Step gave improved sensitivity, which was reflected in lower Ct values. Also, this product is a very good price. Value for money!!" Peony Fung, Cerberus Sciences, Thebarton, Australia

Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions. Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Sensitive – reliable quantification of low abundance targets and scarce samples Fast – delivers reproducible, accurate assay results in as little as 30 minutes Efficient – excellent performance in multiplex assays Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Product Description The SensiFAST™ Probe Lo-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX. SensiFAST Probe has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Probe Kit produces reliable assay results under fast thermal cycling conditions. Furthermore, SensiFAST Probe has been optimized to deliver excellent results in multiplex assays. The SensiFAST Probe Lo-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Sensitive – reliable quantification of low abundance targets and scarce samples Fast – delivers reproducible, accurate assay results in as little as 30 minutes Efficient – excellent performance in multiplex assays Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Product Description The SensiFAST™ Probe No-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX. SensiFAST Probe has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Probe Kit produces reliable assay results under fast thermal cycling conditions. Furthermore, SensiFAST Probe has been optimized to deliver excellent results in multiplex assays. The SensiFAST Probe No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

To complement the SensiFAST™ Probe and SYBR® kits, Bioline has developed the SensiFAST™ cDNA Synthesis Kit to provide a rapid and sensitive method for first-strand cDNA synthesis. The SensiFAST™ cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in real-time PCR studies. A novel, highly-pure reverse transcriptase and new TransAmp™ buffer system delivers highly-efficient synthesis of cDNA, enhanced reproducibility and data accuracy. These features make the SensiFAST™ cDNA Synthesis Kit ideal for working with limited samples, such as laser-micro dissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3’ and 5’ coverage and reverse transcription of all regions. Additionally, the SensiFAST™ cDNA Synthesis Kit can be used with SensiFAST™ Probe and SYBR® Kits for fast real-time RT-PCR without compromising quality, with real-time results in less than an hour.  "Our Institute consisting of more than 60 active researchers has a longstanding interest in understanding changes to the transcriptional landscape of prostate cancer. Reproducibility, sensitivity and cost-effectiveness are critical parameters in selecting our day-to-day cDNA synthesis kit. This combined with its convenient two reagent system continues to make the SensiFast cDNA synthesis kit stand out from its competitors and our preference for almost two years." Martin Sadowski, Queensland University of Technology, Brisbane, Australia

The Tetro cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as analyses of cellular RNAs, characterization of RNA splice variants and the generation and cloning of cDNA.  The Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10 pg-2 μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.

Tetro Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor (fig. 1), with highly efficient and sensitive transcription, from as little as 10 pg, up to 2 μg of RNA (fig. 2). Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures. Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.