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The Tetro cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as analyses of cellular RNAs, characterization of RNA splice variants and the generation and cloning of cDNA. The Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10 pg-2 μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.
To complement the SensiFAST™ Probe and SYBR® kits, Bioline has developed the SensiFAST™ cDNA Synthesis Kit to provide a rapid and sensitive method for first-strand cDNA synthesis. The SensiFAST™ cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in real-time PCR studies. A novel, highly-pure reverse transcriptase and new TransAmp™ buffer system delivers highly-efficient synthesis of cDNA, enhanced reproducibility and data accuracy. These features make the SensiFAST™ cDNA Synthesis Kit ideal for working with limited samples, such as laser-micro dissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3’ and 5’ coverage and reverse transcription of all regions. Additionally, the SensiFAST™ cDNA Synthesis Kit can be used with SensiFAST™ Probe and SYBR® Kits for fast real-time RT-PCR without compromising quality, with real-time results in less than an hour. "Our Institute consisting of more than 60 active researchers has a longstanding interest in understanding changes to the transcriptional landscape of prostate cancer. Reproducibility, sensitivity and cost-effectiveness are critical parameters in selecting our day-to-day cDNA synthesis kit. This combined with its convenient two reagent system continues to make the SensiFast cDNA synthesis kit stand out from its competitors and our preference for almost two years." Martin Sadowski, Queensland University of Technology, Brisbane, Australia
MyTaq™ One-Step RT-PCR Kit has been designed for extremely sensitive and highly reproducible first-stand cDNA synthesis and subsequent PCR in a single tube. The kit contains the latest advances in buffer chemistry, including Bioline ultra-pure dNTPs, together with a proprietary reverse transcriptase and MyTaq HS DNA Polymerase which is our next generation of very high performance, antibody-mediated hot-start DNA polymerase. This ensures that MyTaq One- Step RT-PCR Kit produces fast, highly-specific and ultra-sensitive products for downstream applications. MyTaq One-Step Kit consists of reverse transcriptase, 2x MyTaq HS Mix and the potent RNase Inhibitor RiboSafe, that are blended to create a simple to use all-in-one mix. The kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative, semi-quantitative or quantitative analysis of RNA transcription levels, and the one-step format is also perfect for the synthesis of double stranded cDNA products for subsequent gene expression analysis. The cDNA can be synthesized with starting amounts of RNA template from 3 pg to 1 μg, over a broad temperature range, and up to 50°C to overcome secondary structure and GC-rich sequences), prior to heating to 95°C to inactivate reverse transcriptase and simultaneously to activate the MyTaq™ HS. "When we compared the performance of our routine supplier’s RTase against Bioline’s MyTaq One-Step RT-PCR, the other supplier’s RTase gave two false negatives in five different grapevine samples tested for Grapevine rupestris stem-pitting-associated Foveavirus. We were convinced to immediately switch." University of Adelaide, Australia
Tetro Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor (fig. 1), with highly efficient and sensitive transcription, from as little as 10 pg, up to 2 μg of RNA (fig. 2). Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures. Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.