The SensiFAST Probe Lo-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. SensiFAST Probe One-Step has been optimized to deliver excellent results in both singleplex and multiplex assays. An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation. The SensiFAST™ Probe Lo-ROX One-Step Kit has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX. SensiFAST Probe One-Step has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes. "I compared the SensiFAST Probe One-Step Kit with another very popular product. SensiFAST Probe One-Step gave improved sensitivity, which was reflected in lower Ct values. Also, this product is a very good price. Value for money!!" Peony Fung, Cerberus Sciences, Thebarton, Australia

Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Sensitive – reliable quantification of low abundance targets and scarce samples Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Fast – delivers reproducible, accurate assay results in as little as 30 minutes Product Description The SensiFAST™ SYBR Hi-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that require a high concentration of the passive reference dye ROX. A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR Hi-ROX Kit produces reliable assay results under fast thermal cycling conditions.  An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.  The SensiFAST SYBR® Hi-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Sensitive – reliable quantification of low abundance targets and scarce samples Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Fast – delivers reproducible, accurate assay results in as little as 30 minutes Product Description The SensiFAST™ SYBR Lo-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX. A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR Lo-ROX Kit produces reliable assay results under fast thermal cycling conditions.  An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.  The SensiFAST SYBR® Lo-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Sensitive – reliable quantification of low abundance targets and scarce samples Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Fast – delivers reproducible, accurate assay results in as little as 30 minutes Product Description The SensiFAST™ SYBR No-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX. A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR No-ROX Kit produces reliable assay results under fast thermal cycling conditions.  An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.  The SensiFAST SYBR® No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions. Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Sensitive – reliable quantification of low abundance targets and scarce samples Fast – delivers reproducible, accurate assay results in as little as 30 minutes Efficient – excellent performance in multiplex assays Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Product Description The SensiFAST™ Probe Lo-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX. SensiFAST Probe has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Probe Kit produces reliable assay results under fast thermal cycling conditions. Furthermore, SensiFAST Probe has been optimized to deliver excellent results in multiplex assays. The SensiFAST Probe Lo-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Sensitive – reliable quantification of low abundance targets and scarce samples Fast – delivers reproducible, accurate assay results in as little as 30 minutes Efficient – excellent performance in multiplex assays Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Product Description The SensiFAST™ Probe No-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX. SensiFAST Probe has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Probe Kit produces reliable assay results under fast thermal cycling conditions. Furthermore, SensiFAST Probe has been optimized to deliver excellent results in multiplex assays. The SensiFAST Probe No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

The ISOLATE II Plant DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of plant materials, without the need for hazardous reagents such as phenol. By combining the stringency of CTAB lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plant DNA Kit provides a fast method for the purification of high-quality genomic DNA from most plant cells, particularly those rich in polysaccharides, including leaves, bark, roots and fruits as well as dung, animal-fecal, soil and compost samples. Some plant tissues do not lyse well in CTAB and so an SDS-based lysis buffer is also provided as an alternative. Residual amounts of RNA remaining can be removed using an RNase treatment during lysis with RNase A that is supplied with the kit.  The ISOLATE II Plant DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR with any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase                                     "Isolating DNA from soil samples is a big challenge because samples are full of inhibitors like polymers or low-molecular substances, which inhibit downstream applications. Therefore, it is difficult to find a kit which can deliver stable DNA. The ISOLATE II Plant DNA Kit is able to handle these kinds of samples and worked well for soil samples." Katharina Schulte, Technical College Zweibruecken, Germany

The ISOLATE II Genomic DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of materials, without the need for hazardous reagents such as phenol. By combining Proteinase K lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials, such as mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded tissue (FFPE), stool viruses (e.g. CMV), Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage, EHEC bacteria in food, bacterial DNA (e.g. Chlamydia trachomatis) from cultures, biological fluids or clinical specimens, bacterial DNA (e.g. Borrelia burgdorferi) and viral DNA (e.g. CMV) from urine, insects, dental swabs and buccal swabs. The ISOLATE II Genomic DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.                   "I used the ISOLATE II Genomic DNA Kit to extract genomic DNA from mice tails for genotyping. The kit comes with very clear, user friendly instructions and provides efficient, reliable and cost effective isolation of DNA. Overall a great kit for genomic DNA extraction and downstream analysis." Wilson Wong, University of Sydney, Camperdown, Australia

The ISOLATE II Plasmid Mini Kit provides a simple, efficient column-based method for the isolation of plasmid DNA from bacterial cultures, without the need for hazardous reagents such as phenol. By combining SDS/alkaline lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plasmid Mini Kit provides a fast method for the purification of high-quality plasmid DNA. Separate protocols are provided for the isolation of high-copy plasmids, the isolation of low-copy plasmid, P1 constructs and cosmid DNA from E. coli and the isolation of plasmids from gram-positive bacteria such as Bacillus, Staphylococcus, Bifidobacteria or Corynebacteria. The ISOLATE II Plasmid Mini Kit has been designed to deliver optimal performance in cloning, sequencing as well as end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase. Additionally, the ISOLATE II Plasmid Mini Kit can be used in tandem with the SensiFAST Real-Time PCR Kits for high-performance qPCR.

The ISOLATE II PCR and Gel Kit provides a simple, efficient membrane-based method for the purification of DNA from PCR reactions and from TAE and TBE agarose gels, without the need for hazardous reagents or ethanol precipitation. Six PCR products can be purified in 10 minutes using simple binding and elution steps. Concentrated PCR products ranging between 60 bp and 15 kb can be eluted, removing primers, nucleotides, enzymes, mineral oil, salts and other impurities. DNA fragments between 50 bp and 20 kb can be extracted from six agarose gel slices in about 20 minutes using a color indicator to help maintain optimal pH and identify undissolved agarose. The ISOLATE II PCR and Gel Kit has been designed to deliver optimal performance in transformations, cloning, sequencing and restriction analysis.

The ISOLATE II RNA Mini Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol. By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Mini Kit provides a fast method for the purification of high-quality total RNA from animal and plant cells and tissues as well as cultured cells, bacterial cells, yeast, biological fluids and cell-free samples. Biological samples which are sometimes difficult to process i.e. mouse tissue (liver, brain), various tumor cell lines, Streptococcus and Actinobacillus pleuropneumoniae, will yield high-quality RNA with the ISOLATE II RNA Mini Kit. The online product manual has protocols for purifying total RNA from cultured cells, tissues, yeast, bacteria, biological liquids, paraffin embedded tissue and RNAlater® treated samples. There is also a protocol for a convenient on-column DNase treatment, using RNase-free DNase I that is supplied with the kit, for applications that are sensitive to very small amounts of DNA. The ISOLATE II RNA Mini Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Mini Kit can be used to purify samples for PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.

The ISOLATE II RNA Plant Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of plant materials, including leaves, bark, roots and fruits, without the need for hazardous reagents such as phenol. By combining the stringency of guanidinium thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Plant Kit provides a fast method for the purification of high-quality total RNA from most plant cells, including plant tissues, leaves, bark, roots and fruits. Some plant tissues such as maize endosperm and the mycelia of filamentous fungi do not lyse well in guanidinium thiocyanate and can solidify, resulting in poor yields and so guanidinium hydrochloride lysis buffer is also provided as an alternative. Residual amounts of contaminating DNA are selectively removed using a column, however for ultrasensitive applications, remaining DNA can be removed using RNase-free DNase I that is supplied with the kit.  The ISOLATE II RNA Plant Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Plant Kit can be used to purify samples prior to RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.

The ISOLATE II Biofluid RNA Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol. By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Biofluid RNA Kit provides a fast method for the purification of high-quality total RNA from Biofluids including blood, serum, plasma, urine, saliva and cerebrospinal fluid. A genomic DNA removal column is used to remove contaminating genomic DNA, however for applications that are very sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using DNase treatment with RNase-free DNase I that is supplied with the kit. The ISOLATE II Biofluid RNA Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II Biofluid RNA Kit can be used to purify samples prior to PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.

The ISOLATE II miRNA Kit provides a simple, efficient column-based method for the isolation of miRNA and total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol, which has been reported to introduce significant bias in recovery of selected small RNAs. By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of two silica-membrane purification columns, the ISOLATE II miRNA Kit provides a fast method for the purification of high-quality total RNA (on the first column) and miRNA (on the second column) from cultured animal cells, small tissue samples, bacterial cells, plants and blood. For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment with RNase-free DNase I that is supplied with the kit. The ISOLATE II miRNA Kit has been designed to deliver optimal performance with the EPIK Panel and Select Assays in miRNA expression profiling and quantification using qPCR, microarrays, small RNA library construction for small RNA-Seq, as well as single cell assays.

The ISOLATE II RNA/DNA/Protein Kit provides a simple, efficient column-based method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials, without the need for hazardous reagents such as phenol. By combining the stringency of guanidine-thiocyanate lysis with the speed and ease-of-use of three silica-membrane purification columns, the ISOLATE II RNA/DNA/Protein Kit provides a fast method for the purification of high-quality total RNA, genomic DNA and proteins from a single sample of cultured cells, mammalian tissue, biofluids (including saliva, urine, semen and blood), bacteria, yeast, fungi or plants. For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment with RNase-free DNase I that is supplied with the kit.  The ISOLATE II RNA/DNA/Protein Kit allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population. The purified nucleic acids and proteins are of the highest purity and integrity and the ISOLATE II RNA/DNA/Protein Kit has been designed to deliver optimal performance in a wide variety of downstream applications including miRNA profiling using the EPIK Panel and Select Assays, novel biomarker discovery, siRNA gene silencing studies, gene expression profiling, as well as epigenetics, genotyping, transgenic analysis and cell line characterization.

Many DNA extraction methods can be laborious and time consuming or involve the use of hazardous chemicals. MyTaq™ Extract-PCR Kit offers a rapid, easy and safer alternative for the extraction and amplification of DNA from a variety of tissue types. MyTaq Extract-PCR Kit is particularly suited to solid tissues such as mouse tail or mouse ear. The DNA extractions are performed in a single-tube, without the need for multiple washing steps, greatly reducing the risk of sample loss and contamination. The extracted DNA is amplified in a proprietary buffer system using MyTaq HS Red Mix, the latest generation of very high-performance polymerase unique to Bioline. To further reduce non-specific amplification, MyTaq HS uses antibody hot-start technology. The advanced formulation of MyTaq HS Red Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity or yield. The rapid MyTaq Extract-PCR Kit maximizes sensitivity while minimizing contamination risks to deliver improved success rates in applications such as mouse DNA characterization. The single tube lysis protocol and MyTaq HS Red Mix maximize sensitivity, while minimizing contamination risks and significantly reduce reaction times as well as delivering improved success rates in protocols such as mouse DNA characterization                                                                                                                                                     "We tested the MyTaq Extract-PCR Kit for genotyping mice. The extraction was fast, easy to handle and the PCR reactions worked very well. We used the Taq also for performing multiplex PCR and we obtained better results than with our conventional method." Michael Mitterer, MPI of Immunobiology and Epigenetics, Freiburg

MyTaq™ Plant PCR Kit is recommended for fast, specific and direct PCR from a wide range of plant leaf samples. MyTaq Plant-PCR Kit incorporates MyTaq HS DNA Polymerase, a next-generation hot-start polymerase that delivers highly-specific PCR amplification. Furthermore, MyTaq HS has an increased affinity for template DNA, giving a high PCR product yield for most challenging templates. MyTaq HS DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases meaning it performs reliably even in the presence of PCR inhibitors. MyTaq Plant-PCR Kit includes novel buffer system that replaces the need for complicated extraction or purification steps, including freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction or column DNA purification. The advanced formulation of MyTaq Plant-PCR Kit also allows fast cycling conditions to be used, without compromising PCR specificity and yield. MyTaq Plant-PCR Kit has been developed to tolerate the PCR inhibitors typically present in plant samples, including polyphenolics and polysaccharides, thereby delivering significantly improved assay sensitivity and reproducibility. MyTaq Plant-PCR Kit increases amplification success rates from different plant types and its speed and high specificity makes it ideal for high-throughput genotyping assays. Bioline R&D has obtained successful results when using the MyTaq Plant-PCR Kit to conduct direct PCR from a very broad range of species. These include tomato, rice, sugarcane, maize, potato, soya, wheat, barley, cashew, oak, dwarf umbrella tree, Australian laurel, red-fruit saw-sedge, blueberry lily, she-oaks, coastal rosemary, burrawang, eucalyptus/gum tree, seaweed/crayweed, coastal banksia, bottle brush and honey gem. For more information, please inquire. As part of our test and review campaign, many of our customers have shared their positive experiences of the MyTaq Plant-PCR Kit. Our customers have also reported success for direct PCR from wheat, rosemary, cotton, tobacco, citrus, kangkong, mousear cress (Arabidopsis), cranberry, primrose, blueberry, American bellflower and several grass and fungus species.

HyperLadder™ 1kb is our most popular molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% TAE or TBE agarose gels. The 14 regularly spaced, easy to remember bands, ranging from 200 bp to 10,037 bp, with higher intensity reference bands at 1000 bp and 10,037 bp and a doublet band at 1500/1517 bp allow for easy identification and orientation of your band at a glance. The ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 1kb from the vial to the gel. The approximate mass of DNA in each of the HyperLadder 1kb bands is provided for optionally determining the approximate mass of DNA in comparably intense samples of similar size. The HyperLadder 1kb is also supplied with a 5x loading dye for loading of sample DNA. With a wide size range, HyperLadder 1kb is perfect for size determination in techniques such as sequence analysis, confirmation of plasmid construction and PCR products as well as Southern blotting and other downstream techniques.

HyperLadder™ 100bp is a molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% agarose gels. The 10 regularly spaced bands, ranging from 100 bp to 1013 bp, with a doublet band at 300/311 bp and higher intensity reference bands at 1013 bp allow for easy identification and orientation of your band at a glance. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 100bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 100bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 100bp is also supplied with a 5x loading dye for loading of sample DNA. HyperLadder 100bp is perfect for size determination in techniques such as confirmation of plasmid construction and PCR products as well as other downstream techniques.

HyperLadder™ 50bp is a molecular weight marker, especially designed for easy size determination of linear double-stranded DNA fragments on 2% to 3% TAE or TBE agarose gels. The 10 regularly spaced bands, ranging from 50 bp to 2000 bp, with higher intensity reference bands at 300 bp, 1000 bp and 2000 bp allow for easy identification and orientation of your band at a glance. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 50bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 50bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 50bp is also supplied with a 5x loading dye for loading of sample DNA. HyperLadder 50bp is perfect for size determination in techniques such as confirmation of PCR products as well as other downstream techniques.

HyperLadder™ 25bp is a molecular weight marker, especially designed for easy size determination of linear double-stranded DNA fragments on 2% to 3% TAE or TBE agarose gels or polyacrylamide gels. The 10 regularly spaced bands, ranging from 25 bp to 500 bp, with higher intensity reference bands at 100 bp and 200 bp allow for easy identification and orientation of your band at a glance. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 25bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 25bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 25bp is also supplied with a 5x loading dye for loading of sample DNA. HyperLadder 25bp is perfect for size determination in techniques that produce very short fragments such as apoptotic DNA fragmentation as well as other downstream techniques.

Agarose gels are often run to check and see if a band is present or not, for example in a PCR reaction.  With 5 regularly spaced bands, ranging from 100 bp to 2000 bp, EasyLadder™ I is a molecular weight marker especially designed for fast size determination of linear double-stranded DNA fragments on 0.5% to 3% TAE or TBE agarose gels. This ready-to-use format, with a red loading dye already added to the ladder, reduces handling steps and saves time; simply transfer EasyLadder I from the vial to the gel, along with the PCR product and run at high voltage for just a few minutes (1-3 cm of a gel lane). The concentration of DNA in each of the EasyLadder I bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The EasyLadder I is also supplied with a 5x loading dye for the loading of sample DNA.

HyperPAGE II is a broad range prestained protein marker containing nine highly purified recombinant proteins with molecular weights ranging from 10kDa to 250kDa. The proteins are prestained with several fluorescent dyes and resolve into sharp, clearly identifiable bands for easy visualization and orientation. The green 10kDa, orange 70kDa and pink 140kDa bands serve as useful reference bands. HyperPAGE II is suitable to monitor migration efficiency in SDS-PAGE and protein transfer from gel to membrane in Western blotting, without the addition of staining dye. Prestained protein markers are suitable for determining the approximate molecular weights of proteins.   

Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is ideal for isolating PCR and RT-PCR templates. The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 μg/mL for protein removal and enzyme inactivation and up to 2 mg/mL for tissue treatment. Proteinase K products are free of detectable DNase and RNase.

Product Highlights Ready-to-use - no need to add dye Dye visible in gel - allow users to monitor DNA migration Guaranteed batch-to-batch reproducibility - provides consistent results Product Description DNA Loading Buffer Blue is one of a range of Bioline Colored DNA Loading Buffers (fig. 1). The ready-to-use solution is premixed with bromophenol blue that migrate at different rates depending on the dye (fig.1 Lane 3) and the concentration of the agarose gel (see Dye Migration Table). This allow you to monitor DNA migration and therefore increase the versatility of your DNA analysis.

TRIsure provides a simple, efficient method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials. By combining thiocyanate compounds with phenol, TRIsure facilitates disruption of cells during homogenization and effectively inhibits DNase and RNase activity. After homogenizing the sample with TRIsure, chloroform is added and the homogenate is allowed to separate into three phases: a clear aqueous phase (upper) containing the RNA, a green colored organic phase (lower) and an interphase, containing the DNA and proteins. The RNA is extracted from the aqueous phase by isopropyl alcohol precipitation, DNA is precipitated from the organic layer with ethanol and the proteins are precipitated from the phenol-ethanol supernatant by isopropyl alcohol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for use in downstream applications. TRIsure allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population. The simplicity of the TRIsure method allows simultaneous processing of a large number of samples and can be used in a wide variety of downstream applications including expression profiling, epigenetics, genotyping, transgenic analysis and cell line characterization.

Highly quality dNTPs are a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Bioline ultra-pure dNTPs undergo highly stringent purification steps to give a greater than 99% purity and are tested or the absence of DNase, RNase, Protease, Nickase activity. They are then quality controlled& in a variety of applications, ensuring that they are highly suitable for the most sensitive of PCR techniques. All Bioline dNTP Mixes are supplied as an equimolar solution of ultra-pure dATP, dCTP, dGTP and dTTP as lithium salts. Lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life. As an ISO 13485 certified primary manufacturer of nucleotides, if your requirements for nucleotides are beyond the scope of our standard product range, Bioline can also offer custom, bulk and OEM services (custom@bioline.com).

Product Highlights Excellent clarity - enhances visualization, even at high concentrations Strong gels at low concentration - better handling, less breakage DNase/RNase-free - for all nucleic acid separation Product Description Extremely pure, high molecular biology grade Agarose from Bioline has no detectable DNase or RNase activity and forms strong gels with low backgrounds. Due to its low EEO, DNA will have a high electrophoretic mobility.

Product Highlights Long oligonucleotide -more thermostable Reverse Transcriptases perform better with longer primers Binds only to mRNA -can be used for cDNA synthesis with total RNA Product Description Oligo (dT)18 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)18 ensures that the 3´ end of mRNAs are represented. Primer sequence: 5´-d (TTT TTT TTT TTT TTT TTT)-3´ A mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.

Product Highlights Induces E.coli lac operon activity - suitable for cloning and protein expression procedures Dioxane free - does not disrupt normal cell function >99.6% purity - confirmed by HPLC Convenient - available as powder and stabilized stock solution Product Description Isopropyl-ß-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme ß--Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG. IPTG is part of the Bioline Essentials range of products, which includes agaroses, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.

Fully autoclavable, Ergonomic design maximizing user comfort, Easy-to-read volume display, Easy calibration and maintenance,

เป็น 8-channel pipette ใช้ในการดูดจ่ายสารละลาย สามารถนึ่งฆ่าเชื้อได้ทั้งตัว ใช้ร่วมกับทิปได้หลายยี่ห้อ ผลิตภัณฑ์จาก USA มีบริการ calibration โดยบริษัทรับสอบเทียบที่ได้รับการรับรองมาตรฐาน ISO17025

เป็น 12-channel pipette ใช้ในการดูดจ่ายสารละลาย สามารถนึ่งฆ่าเชื้อได้ทั้งตัว ใช้ร่วมกับทิปได้หลายยี่ห้อ ผลิตภัณฑ์จาก USA มีบริการ calibration โดยบริษัทรับสอบเทียบที่ได้รับการรับรองมาตรฐาน ISO17025

Appropriate for most of the plastic and glass pipettes from 0.1-100ml, Eight speeds of selection for aspiration and dispensing different liquids, Large LCD Display showing low battery warning and speed settings, Enabling single-handed operation with minimun effort, High capacity Li-on battery enable long runtime of operation, 0.45um replaceable Hydrophobic filter.

Enabling single-handed operation with minimun effort, Appropriate for most of the plastic and glass from 0.1-100ml, Easily adjusted speed control, 0.45um replaceable Hydrophobic filter, Stable and reliable preformance, elegant apperance, High Capacity Li-on battery enable long runtime of operation

Ease of use, precise pipetting control, Robust and lightweight, Suitable for 0.1-100ml volumetric or serological pipettes, Filling and delivery comfortably achieved with a sensitive lever, for integral or replacement filter, 3um, easy cleaning and maintenancen, autocalvable silicone pipette holder.

Light and ergonomic solution provide easy usability, Single-handed operation, Volume range fron 1ul to 5ml, Up to 48 dispensing steps, Maintenance free, Equipped with durable tip insertion lever, Fits seven sizes of disposable polypropylene syringes from 0.5ml to 50ml.

High quality motor providing excellent precision and accuracy, Electronic control decreases the repetitive strain injury, the remove control panel makes the operating process without the manual disturbance, Build-in magnetic stirrer support.

Motor operation minimizes the grip force, Electronic control decreases the repetitive strain injury, the remote control panel makes the operating process without the manual disturbance, Two disoensing modes, dispenser and stepper function options.

            Excellent chemical resistance, components are made of (PTFE, FEP, BSG, PP), Fully autoclavable at 121C, Four ranges of bottle-top dispenser covering a volume range from 0.5ml to 50ml, Easy for cleaning and maintenance, the Optional flexible discharge tube with safety handle permits fast and precise dispensing, Dispensemate is supplied with S40, GL32, GL38, GL25,GL28 sized adapters sized adaptors.

Vacuum power can be set to different levels to achieve 1ml/s to 15ml/s aspirating speed, Sensitive level sensor to prevent liquid overflow during operation, Waste bottle, tubing and handle are fully autoclavable, Lid, tubing and handle are easy for disassembling, cleaning and autoclaving, Wide range of adaptors to fit tube, microplate and disk plate for various tasks

Reliable heating/cooling elements and innovative temperature control, High performance DSP and temperature control, Excellent temperature uniformity, high precise temperature control, Fast heating and cooling rate, 7 inch Large color touch panel screen for easy programming, large user program storage.

Reliable heating/cooling elements and innovative temperature control, High performance DSP and temperature control, Excellent temperature uniformity, high precise temperature control, Fast heating and cooling rate, 7 inch Large color touch panel screen for easy programming, large user program storage.

Digital display for both rotation speed and heating temperature allows for optimal controlling of all distillation processes, Motor lift automatic release the evaporating flask to a safety position in case of power failure, Wide temperature range of the 5ml heating bath(Room temperature 180C) Overheating protection temperature at 220C, Speed range from 20 to 280 rpm,. and inteval operation in clockwise and anticlockwise directions for drying process, Patented condenser with excellent cooling effect, ejection mechanism ensures easy removal of evaporating flask, patented double spring sealing ring which made of PTFE provides an excellent sealing performance, Remote function provides PC control and data transmission.

LCD Display for settings and monitoring of speed and temperature, Bult-in PID controller ensures the safe heating of the medium with overheating protection, glass ceramic work plate provides excellent chemical resistant performance and most eifficient heat transfer, Outstanding brushless DC motor performance enables more stirring power, two rotating knobs enable the easy ane direct chage of speed and temperature

LED Display for precise monitoring of temperature, a wide speed range of 0 to 1500rpm

Brushless DC motor in maintenance free and explosion-proof, Digital temperature control max. temperature at 340C, Digital speed control with Max. speed up to 1500rpm, Safety circuits provides overheating protection, wide range timer function from 1min to 99hr59min, The high-resolution LCD Display shows actual temperatures and speed, a wide variety of accessories are available