MyTaq™ HS Red DNA Polymerase is recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. The MyTaq HS DNA Polymerase and MyTaq Red Reaction Buffer in this product, are a unique combination of next-generation hot-start DNA polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq HS has been developed to give more robust amplification than other commonly-used polymerases meaning it performs reliably even in the presence of PCR inhibitors MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme, specifically designed for highly-specific, efficient amplification from even the most challenging templates. MyTaq HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start. Furthermore, the advanced formulation of MyTaq HS and buffer system allows fast cycling conditions, considerably reducing the reaction time without compromising PCR specificity or yield. The enzyme is supplied with a 5x MyTaq Red Reaction Buffer that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, the MyTaq Red Reaction Buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

MyTaq™ Red Mix is recommended for all standard PCR applications. MyTaq Red Mix is comprised of MyTaq DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq means it gives excellent results under fast PCR conditions. The product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up, for improved speed, throughput and assay reproducibility. MyTaq Red Mix contains a red dye that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, the MyTaq red reaction buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

MyTaq HS Red Mix is recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. MyTaq HS Red Mix is comprised of MyTaq HS DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq HS has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors.  Furthermore, the highly efficient nature of MyTaq HS means it gives excellent results under fast PCR conditions. MyTaq HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start. The product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up, for improved speed, throughput and assay reproducibility. MyTaq Red Mix contains a red dye that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, MyTaq Red contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

To complement the SensiFAST™ Probe and SYBR® kits, Bioline has developed the SensiFAST™ cDNA Synthesis Kit to provide a rapid and sensitive method for first-strand cDNA synthesis. The SensiFAST™ cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in real-time PCR studies. A novel, highly-pure reverse transcriptase and new TransAmp™ buffer system delivers highly-efficient synthesis of cDNA, enhanced reproducibility and data accuracy. These features make the SensiFAST™ cDNA Synthesis Kit ideal for working with limited samples, such as laser-micro dissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3’ and 5’ coverage and reverse transcription of all regions. Additionally, the SensiFAST™ cDNA Synthesis Kit can be used with SensiFAST™ Probe and SYBR® Kits for fast real-time RT-PCR without compromising quality, with real-time results in less than an hour.  "Our Institute consisting of more than 60 active researchers has a longstanding interest in understanding changes to the transcriptional landscape of prostate cancer. Reproducibility, sensitivity and cost-effectiveness are critical parameters in selecting our day-to-day cDNA synthesis kit. This combined with its convenient two reagent system continues to make the SensiFast cDNA synthesis kit stand out from its competitors and our preference for almost two years." Martin Sadowski, Queensland University of Technology, Brisbane, Australia

The ISOLATE II Plant DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of plant materials, without the need for hazardous reagents such as phenol. By combining the stringency of CTAB lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plant DNA Kit provides a fast method for the purification of high-quality genomic DNA from most plant cells, particularly those rich in polysaccharides, including leaves, bark, roots and fruits as well as dung, animal-fecal, soil and compost samples. Some plant tissues do not lyse well in CTAB and so an SDS-based lysis buffer is also provided as an alternative. Residual amounts of RNA remaining can be removed using an RNase treatment during lysis with RNase A that is supplied with the kit.  The ISOLATE II Plant DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR with any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase                                     "Isolating DNA from soil samples is a big challenge because samples are full of inhibitors like polymers or low-molecular substances, which inhibit downstream applications. Therefore, it is difficult to find a kit which can deliver stable DNA. The ISOLATE II Plant DNA Kit is able to handle these kinds of samples and worked well for soil samples." Katharina Schulte, Technical College Zweibruecken, Germany

The ISOLATE II Genomic DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of materials, without the need for hazardous reagents such as phenol. By combining Proteinase K lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials, such as mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded tissue (FFPE), stool viruses (e.g. CMV), Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage, EHEC bacteria in food, bacterial DNA (e.g. Chlamydia trachomatis) from cultures, biological fluids or clinical specimens, bacterial DNA (e.g. Borrelia burgdorferi) and viral DNA (e.g. CMV) from urine, insects, dental swabs and buccal swabs. The ISOLATE II Genomic DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.                   "I used the ISOLATE II Genomic DNA Kit to extract genomic DNA from mice tails for genotyping. The kit comes with very clear, user friendly instructions and provides efficient, reliable and cost effective isolation of DNA. Overall a great kit for genomic DNA extraction and downstream analysis." Wilson Wong, University of Sydney, Camperdown, Australia

The ISOLATE II Plasmid Mini Kit provides a simple, efficient column-based method for the isolation of plasmid DNA from bacterial cultures, without the need for hazardous reagents such as phenol. By combining SDS/alkaline lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plasmid Mini Kit provides a fast method for the purification of high-quality plasmid DNA. Separate protocols are provided for the isolation of high-copy plasmids, the isolation of low-copy plasmid, P1 constructs and cosmid DNA from E. coli and the isolation of plasmids from gram-positive bacteria such as Bacillus, Staphylococcus, Bifidobacteria or Corynebacteria. The ISOLATE II Plasmid Mini Kit has been designed to deliver optimal performance in cloning, sequencing as well as end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase. Additionally, the ISOLATE II Plasmid Mini Kit can be used in tandem with the SensiFAST Real-Time PCR Kits for high-performance qPCR.

The ISOLATE II PCR and Gel Kit provides a simple, efficient membrane-based method for the purification of DNA from PCR reactions and from TAE and TBE agarose gels, without the need for hazardous reagents or ethanol precipitation. Six PCR products can be purified in 10 minutes using simple binding and elution steps. Concentrated PCR products ranging between 60 bp and 15 kb can be eluted, removing primers, nucleotides, enzymes, mineral oil, salts and other impurities. DNA fragments between 50 bp and 20 kb can be extracted from six agarose gel slices in about 20 minutes using a color indicator to help maintain optimal pH and identify undissolved agarose. The ISOLATE II PCR and Gel Kit has been designed to deliver optimal performance in transformations, cloning, sequencing and restriction analysis.

The ISOLATE II RNA Mini Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol. By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Mini Kit provides a fast method for the purification of high-quality total RNA from animal and plant cells and tissues as well as cultured cells, bacterial cells, yeast, biological fluids and cell-free samples. Biological samples which are sometimes difficult to process i.e. mouse tissue (liver, brain), various tumor cell lines, Streptococcus and Actinobacillus pleuropneumoniae, will yield high-quality RNA with the ISOLATE II RNA Mini Kit. The online product manual has protocols for purifying total RNA from cultured cells, tissues, yeast, bacteria, biological liquids, paraffin embedded tissue and RNAlater® treated samples. There is also a protocol for a convenient on-column DNase treatment, using RNase-free DNase I that is supplied with the kit, for applications that are sensitive to very small amounts of DNA. The ISOLATE II RNA Mini Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Mini Kit can be used to purify samples for PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.

The ISOLATE II RNA Plant Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of plant materials, including leaves, bark, roots and fruits, without the need for hazardous reagents such as phenol. By combining the stringency of guanidinium thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Plant Kit provides a fast method for the purification of high-quality total RNA from most plant cells, including plant tissues, leaves, bark, roots and fruits. Some plant tissues such as maize endosperm and the mycelia of filamentous fungi do not lyse well in guanidinium thiocyanate and can solidify, resulting in poor yields and so guanidinium hydrochloride lysis buffer is also provided as an alternative. Residual amounts of contaminating DNA are selectively removed using a column, however for ultrasensitive applications, remaining DNA can be removed using RNase-free DNase I that is supplied with the kit.  The ISOLATE II RNA Plant Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Plant Kit can be used to purify samples prior to RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.

The ISOLATE II Biofluid RNA Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol. By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Biofluid RNA Kit provides a fast method for the purification of high-quality total RNA from Biofluids including blood, serum, plasma, urine, saliva and cerebrospinal fluid. A genomic DNA removal column is used to remove contaminating genomic DNA, however for applications that are very sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using DNase treatment with RNase-free DNase I that is supplied with the kit. The ISOLATE II Biofluid RNA Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II Biofluid RNA Kit can be used to purify samples prior to PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.

The ISOLATE II miRNA Kit provides a simple, efficient column-based method for the isolation of miRNA and total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol, which has been reported to introduce significant bias in recovery of selected small RNAs. By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of two silica-membrane purification columns, the ISOLATE II miRNA Kit provides a fast method for the purification of high-quality total RNA (on the first column) and miRNA (on the second column) from cultured animal cells, small tissue samples, bacterial cells, plants and blood. For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment with RNase-free DNase I that is supplied with the kit. The ISOLATE II miRNA Kit has been designed to deliver optimal performance with the EPIK Panel and Select Assays in miRNA expression profiling and quantification using qPCR, microarrays, small RNA library construction for small RNA-Seq, as well as single cell assays.

The ISOLATE II RNA/DNA/Protein Kit provides a simple, efficient column-based method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials, without the need for hazardous reagents such as phenol. By combining the stringency of guanidine-thiocyanate lysis with the speed and ease-of-use of three silica-membrane purification columns, the ISOLATE II RNA/DNA/Protein Kit provides a fast method for the purification of high-quality total RNA, genomic DNA and proteins from a single sample of cultured cells, mammalian tissue, biofluids (including saliva, urine, semen and blood), bacteria, yeast, fungi or plants. For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment with RNase-free DNase I that is supplied with the kit.  The ISOLATE II RNA/DNA/Protein Kit allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population. The purified nucleic acids and proteins are of the highest purity and integrity and the ISOLATE II RNA/DNA/Protein Kit has been designed to deliver optimal performance in a wide variety of downstream applications including miRNA profiling using the EPIK Panel and Select Assays, novel biomarker discovery, siRNA gene silencing studies, gene expression profiling, as well as epigenetics, genotyping, transgenic analysis and cell line characterization.

Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is ideal for isolating PCR and RT-PCR templates. The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 μg/mL for protein removal and enzyme inactivation and up to 2 mg/mL for tissue treatment. Proteinase K products are free of detectable DNase and RNase.

Product Highlights Ready-to-use - no need to add dye Dye visible in gel - allow users to monitor DNA migration Guaranteed batch-to-batch reproducibility - provides consistent results Product Description DNA Loading Buffer Blue is one of a range of Bioline Colored DNA Loading Buffers (fig. 1). The ready-to-use solution is premixed with bromophenol blue that migrate at different rates depending on the dye (fig.1 Lane 3) and the concentration of the agarose gel (see Dye Migration Table). This allow you to monitor DNA migration and therefore increase the versatility of your DNA analysis.

TRIsure provides a simple, efficient method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials. By combining thiocyanate compounds with phenol, TRIsure facilitates disruption of cells during homogenization and effectively inhibits DNase and RNase activity. After homogenizing the sample with TRIsure, chloroform is added and the homogenate is allowed to separate into three phases: a clear aqueous phase (upper) containing the RNA, a green colored organic phase (lower) and an interphase, containing the DNA and proteins. The RNA is extracted from the aqueous phase by isopropyl alcohol precipitation, DNA is precipitated from the organic layer with ethanol and the proteins are precipitated from the phenol-ethanol supernatant by isopropyl alcohol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for use in downstream applications. TRIsure allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population. The simplicity of the TRIsure method allows simultaneous processing of a large number of samples and can be used in a wide variety of downstream applications including expression profiling, epigenetics, genotyping, transgenic analysis and cell line characterization.

Highly quality dNTPs are a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Bioline ultra-pure dNTPs undergo highly stringent purification steps to give a greater than 99% purity and are tested or the absence of DNase, RNase, Protease, Nickase activity. They are then quality controlled& in a variety of applications, ensuring that they are highly suitable for the most sensitive of PCR techniques. All Bioline dNTP Mixes are supplied as an equimolar solution of ultra-pure dATP, dCTP, dGTP and dTTP as lithium salts. Lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life. As an ISO 13485 certified primary manufacturer of nucleotides, if your requirements for nucleotides are beyond the scope of our standard product range, Bioline can also offer custom, bulk and OEM services (custom@bioline.com).

Product Highlights Excellent clarity - enhances visualization, even at high concentrations Strong gels at low concentration - better handling, less breakage DNase/RNase-free - for all nucleic acid separation Product Description Extremely pure, high molecular biology grade Agarose from Bioline has no detectable DNase or RNase activity and forms strong gels with low backgrounds. Due to its low EEO, DNA will have a high electrophoretic mobility.

Product Highlights Long oligonucleotide -more thermostable Reverse Transcriptases perform better with longer primers Binds only to mRNA -can be used for cDNA synthesis with total RNA Product Description Oligo (dT)18 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)18 ensures that the 3´ end of mRNAs are represented. Primer sequence: 5´-d (TTT TTT TTT TTT TTT TTT)-3´ A mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.

Product Highlights Induces E.coli lac operon activity - suitable for cloning and protein expression procedures Dioxane free - does not disrupt normal cell function >99.6% purity - confirmed by HPLC Convenient - available as powder and stabilized stock solution Product Description Isopropyl-ß-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme ß--Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG. IPTG is part of the Bioline Essentials range of products, which includes agaroses, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.