Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is ideal for isolating PCR and RT-PCR templates. The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 μg/mL for protein removal and enzyme inactivation and up to 2 mg/mL for tissue treatment. Proteinase K products are free of detectable DNase and RNase.

Product Highlights Ready-to-use - no need to add dye Dye visible in gel - allow users to monitor DNA migration Guaranteed batch-to-batch reproducibility - provides consistent results Product Description DNA Loading Buffer Blue is one of a range of Bioline Colored DNA Loading Buffers (fig. 1). The ready-to-use solution is premixed with bromophenol blue that migrate at different rates depending on the dye (fig.1 Lane 3) and the concentration of the agarose gel (see Dye Migration Table). This allow you to monitor DNA migration and therefore increase the versatility of your DNA analysis.

TRIsure provides a simple, efficient method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials. By combining thiocyanate compounds with phenol, TRIsure facilitates disruption of cells during homogenization and effectively inhibits DNase and RNase activity. After homogenizing the sample with TRIsure, chloroform is added and the homogenate is allowed to separate into three phases: a clear aqueous phase (upper) containing the RNA, a green colored organic phase (lower) and an interphase, containing the DNA and proteins. The RNA is extracted from the aqueous phase by isopropyl alcohol precipitation, DNA is precipitated from the organic layer with ethanol and the proteins are precipitated from the phenol-ethanol supernatant by isopropyl alcohol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for use in downstream applications. TRIsure allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population. The simplicity of the TRIsure method allows simultaneous processing of a large number of samples and can be used in a wide variety of downstream applications including expression profiling, epigenetics, genotyping, transgenic analysis and cell line characterization.

Highly quality dNTPs are a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Bioline ultra-pure dNTPs undergo highly stringent purification steps to give a greater than 99% purity and are tested or the absence of DNase, RNase, Protease, Nickase activity. They are then quality controlled& in a variety of applications, ensuring that they are highly suitable for the most sensitive of PCR techniques. All Bioline dNTP Mixes are supplied as an equimolar solution of ultra-pure dATP, dCTP, dGTP and dTTP as lithium salts. Lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life. As an ISO 13485 certified primary manufacturer of nucleotides, if your requirements for nucleotides are beyond the scope of our standard product range, Bioline can also offer custom, bulk and OEM services (custom@bioline.com).

Product Highlights Excellent clarity - enhances visualization, even at high concentrations Strong gels at low concentration - better handling, less breakage DNase/RNase-free - for all nucleic acid separation Product Description Extremely pure, high molecular biology grade Agarose from Bioline has no detectable DNase or RNase activity and forms strong gels with low backgrounds. Due to its low EEO, DNA will have a high electrophoretic mobility.

Product Highlights Long oligonucleotide -more thermostable Reverse Transcriptases perform better with longer primers Binds only to mRNA -can be used for cDNA synthesis with total RNA Product Description Oligo (dT)18 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)18 ensures that the 3´ end of mRNAs are represented. Primer sequence: 5´-d (TTT TTT TTT TTT TTT TTT)-3´ A mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.

Product Highlights Induces E.coli lac operon activity - suitable for cloning and protein expression procedures Dioxane free - does not disrupt normal cell function >99.6% purity - confirmed by HPLC Convenient - available as powder and stabilized stock solution Product Description Isopropyl-ß-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme ß--Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG. IPTG is part of the Bioline Essentials range of products, which includes agaroses, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.

Highly quality dNTPs are a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Bioline ultra-pure dNTPs undergo highly stringent purification steps to give a greater than 99% purity and are tested or the absence of DNase, RNase, Protease, Nickase activity. They are then quality controlled& in a variety of applications, ensuring that they are highly suitable for the most sensitive of PCR techniques. All Bioline dNTP Mixes are supplied as an equimolar solution of ultra-pure dATP, dCTP, dGTP and dTTP as lithium salts. Lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life. As an ISO 13485 certified primary manufacturer of nucleotides, if your requirements for nucleotides are beyond the scope of our standard product range, Bioline can also offer custom, bulk and OEM services (custom@bioline.com).

The ready-to-use molecular grade dNTP Sets consist of four separate 100 mM solutions of dATP, dCTP, dGTP and dTTP respectively. Product Highlights High quality - >99% purity determined by HPLC, ideal for use in PCR reactions Long shelf-life - no need for aliquoting Convenient formats - available in various concentrations, offering ease of use Enzyme free - DNase, RNase and Nickase free Flexible sizes - custom, bulk and OEM sizes available Applications DNA amplification (standard, long-range, multiplex and high-fidelity PCR) DNA labeling RT-PCR qPCR Genotyping cDNA synthesis DNA sequencing

Many DNA extraction methods can be laborious and time consuming or involve the use of hazardous chemicals. MyTaq™ Extract-PCR Kit offers a rapid, easy and safer alternative for the extraction and amplification of DNA from a variety of tissue types. MyTaq Extract-PCR Kit is particularly suited to solid tissues such as mouse tail or mouse ear. The DNA extractions are performed in a single-tube, without the need for multiple washing steps, greatly reducing the risk of sample loss and contamination. The extracted DNA is amplified in a proprietary buffer system using MyTaq HS Red Mix, the latest generation of very high-performance polymerase unique to Bioline. To further reduce non-specific amplification, MyTaq HS uses antibody hot-start technology. The advanced formulation of MyTaq HS Red Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity or yield. The rapid MyTaq Extract-PCR Kit maximizes sensitivity while minimizing contamination risks to deliver improved success rates in applications such as mouse DNA characterization. The single tube lysis protocol and MyTaq HS Red Mix maximize sensitivity, while minimizing contamination risks and significantly reduce reaction times as well as delivering improved success rates in protocols such as mouse DNA characterization                                                                                                                                                     "We tested the MyTaq Extract-PCR Kit for genotyping mice. The extraction was fast, easy to handle and the PCR reactions worked very well. We used the Taq also for performing multiplex PCR and we obtained better results than with our conventional method." Michael Mitterer, MPI of Immunobiology and Epigenetics, Freiburg

MyTaq™ Plant PCR Kit is recommended for fast, specific and direct PCR from a wide range of plant leaf samples. MyTaq Plant-PCR Kit incorporates MyTaq HS DNA Polymerase, a next-generation hot-start polymerase that delivers highly-specific PCR amplification. Furthermore, MyTaq HS has an increased affinity for template DNA, giving a high PCR product yield for most challenging templates. MyTaq HS DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases meaning it performs reliably even in the presence of PCR inhibitors. MyTaq Plant-PCR Kit includes novel buffer system that replaces the need for complicated extraction or purification steps, including freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction or column DNA purification. The advanced formulation of MyTaq Plant-PCR Kit also allows fast cycling conditions to be used, without compromising PCR specificity and yield. MyTaq Plant-PCR Kit has been developed to tolerate the PCR inhibitors typically present in plant samples, including polyphenolics and polysaccharides, thereby delivering significantly improved assay sensitivity and reproducibility. MyTaq Plant-PCR Kit increases amplification success rates from different plant types and its speed and high specificity makes it ideal for high-throughput genotyping assays. Bioline R&D has obtained successful results when using the MyTaq Plant-PCR Kit to conduct direct PCR from a very broad range of species. These include tomato, rice, sugarcane, maize, potato, soya, wheat, barley, cashew, oak, dwarf umbrella tree, Australian laurel, red-fruit saw-sedge, blueberry lily, she-oaks, coastal rosemary, burrawang, eucalyptus/gum tree, seaweed/crayweed, coastal banksia, bottle brush and honey gem. For more information, please inquire. As part of our test and review campaign, many of our customers have shared their positive experiences of the MyTaq Plant-PCR Kit. Our customers have also reported success for direct PCR from wheat, rosemary, cotton, tobacco, citrus, kangkong, mousear cress (Arabidopsis), cranberry, primrose, blueberry, American bellflower and several grass and fungus species.

HyperLadder™ 1kb is our most popular molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% TAE or TBE agarose gels. The 14 regularly spaced, easy to remember bands, ranging from 200 bp to 10,037 bp, with higher intensity reference bands at 1000 bp and 10,037 bp and a doublet band at 1500/1517 bp allow for easy identification and orientation of your band at a glance. The ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 1kb from the vial to the gel. The approximate mass of DNA in each of the HyperLadder 1kb bands is provided for optionally determining the approximate mass of DNA in comparably intense samples of similar size. The HyperLadder 1kb is also supplied with a 5x loading dye for loading of sample DNA. With a wide size range, HyperLadder 1kb is perfect for size determination in techniques such as sequence analysis, confirmation of plasmid construction and PCR products as well as Southern blotting and other downstream techniques.

HyperLadder™ 100bp is a molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% agarose gels. The 10 regularly spaced bands, ranging from 100 bp to 1013 bp, with a doublet band at 300/311 bp and higher intensity reference bands at 1013 bp allow for easy identification and orientation of your band at a glance. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 100bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 100bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 100bp is also supplied with a 5x loading dye for loading of sample DNA. HyperLadder 100bp is perfect for size determination in techniques such as confirmation of plasmid construction and PCR products as well as other downstream techniques.

HyperLadder™ 50bp is a molecular weight marker, especially designed for easy size determination of linear double-stranded DNA fragments on 2% to 3% TAE or TBE agarose gels. The 10 regularly spaced bands, ranging from 50 bp to 2000 bp, with higher intensity reference bands at 300 bp, 1000 bp and 2000 bp allow for easy identification and orientation of your band at a glance. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 50bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 50bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 50bp is also supplied with a 5x loading dye for loading of sample DNA. HyperLadder 50bp is perfect for size determination in techniques such as confirmation of PCR products as well as other downstream techniques.

HyperLadder™ 25bp is a molecular weight marker, especially designed for easy size determination of linear double-stranded DNA fragments on 2% to 3% TAE or TBE agarose gels or polyacrylamide gels. The 10 regularly spaced bands, ranging from 25 bp to 500 bp, with higher intensity reference bands at 100 bp and 200 bp allow for easy identification and orientation of your band at a glance. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 25bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 25bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 25bp is also supplied with a 5x loading dye for loading of sample DNA. HyperLadder 25bp is perfect for size determination in techniques that produce very short fragments such as apoptotic DNA fragmentation as well as other downstream techniques.

Agarose gels are often run to check and see if a band is present or not, for example in a PCR reaction.  With 5 regularly spaced bands, ranging from 100 bp to 2000 bp, EasyLadder™ I is a molecular weight marker especially designed for fast size determination of linear double-stranded DNA fragments on 0.5% to 3% TAE or TBE agarose gels. This ready-to-use format, with a red loading dye already added to the ladder, reduces handling steps and saves time; simply transfer EasyLadder I from the vial to the gel, along with the PCR product and run at high voltage for just a few minutes (1-3 cm of a gel lane). The concentration of DNA in each of the EasyLadder I bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The EasyLadder I is also supplied with a 5x loading dye for the loading of sample DNA.

HyperPAGE II is a broad range prestained protein marker containing nine highly purified recombinant proteins with molecular weights ranging from 10kDa to 250kDa. The proteins are prestained with several fluorescent dyes and resolve into sharp, clearly identifiable bands for easy visualization and orientation. The green 10kDa, orange 70kDa and pink 140kDa bands serve as useful reference bands. HyperPAGE II is suitable to monitor migration efficiency in SDS-PAGE and protein transfer from gel to membrane in Western blotting, without the addition of staining dye. Prestained protein markers are suitable for determining the approximate molecular weights of proteins.   

The ISOLATE II Plant DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of plant materials, without the need for hazardous reagents such as phenol. By combining the stringency of CTAB lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plant DNA Kit provides a fast method for the purification of high-quality genomic DNA from most plant cells, particularly those rich in polysaccharides, including leaves, bark, roots and fruits as well as dung, animal-fecal, soil and compost samples. Some plant tissues do not lyse well in CTAB and so an SDS-based lysis buffer is also provided as an alternative. Residual amounts of RNA remaining can be removed using an RNase treatment during lysis with RNase A that is supplied with the kit.  The ISOLATE II Plant DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR with any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase                                     "Isolating DNA from soil samples is a big challenge because samples are full of inhibitors like polymers or low-molecular substances, which inhibit downstream applications. Therefore, it is difficult to find a kit which can deliver stable DNA. The ISOLATE II Plant DNA Kit is able to handle these kinds of samples and worked well for soil samples." Katharina Schulte, Technical College Zweibruecken, Germany